Application
ChIP was performed with U2OS nuclear extract and 1.5 μg of MBD1 antibody. Beads only were used as a negative IP control. The precipitated DNA was detected by QPCR with primer set targeting to GAPDH promoter or myoglobin.Application
WB analysis of nuclear extracts (40 μg) of HeLa cells using MBD1 antibody diluted 1:500 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of MBD1 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.| Product Name | MBD1 antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MBD1 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWBChIP |
| ChIP (1.5 µg), ELISA (1:1,000), Western blotting (1:500): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PCM1 RFT methyl-CpG binding domain protein 1 CXXC3 |
|---|---|
| Target | MBD1 |
Application
ChIP was performed with U2OS nuclear extract and 1.5 μg of MBD1 antibody. Beads only were used as a negative IP control. The precipitated DNA was detected by QPCR with primer set targeting to GAPDH promoter or myoglobin.
Application
WB analysis of nuclear extracts (40 μg) of HeLa cells using MBD1 antibody diluted 1:500 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of MBD1 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.| Application Notes | ChIP (1.5 µg), ELISA (1:1,000), Western blotting (1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
