Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PCM-1 antibody in Ramos cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Fluorescent image of A549 cell stained with PCM-1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7unit| Product Name | PCM-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1 |
| Product description | PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.1) Balczon R., Bao L.J. Cell Biol. 124:783-793(1994) |
| Immunogen | This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 632-661 amino acids from the Central region of human PCM-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PCM1 |
|---|---|
| Alternative Names | Pericentriolar material 1 protein PCM-1 hPCM-1 PCM1 |
| Molecular Weight(MW) | 229 kDa |
Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PCM-1 antibody in Ramos cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Fluorescent image of A549 cell stained with PCM-1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7unit| Application Notes | For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
