WB
Western blot analysis of PCM1 using anti-PCM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PCM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PCM1 using anti-PCM1 antibody. PCM1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PCM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PCM1 using anti-PCM1 antibody. PCM1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PCM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PCM1 using anti-PCM1 antibody. PCM1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PCM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to PCM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PCM1 recombinant protein (Position: M1-K259). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCM1 |
|---|---|
| Gene Synonyms | PTC4 RET/PCM-1 |
| Gene Full Name | pericentriolar material 1 |
| Gene Summary | The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] |
| Alternative Names | Pericentriolar material 1 protein PCM-1 hPCM-1 PCM1 |
| Molecular Weight(MW) | 229 kDa(Observed band size:280 kDa) |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton. |
WB
Western blot analysis of PCM1 using anti-PCM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PCM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PCM1 using anti-PCM1 antibody. PCM1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PCM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PCM1 using anti-PCM1 antibody. PCM1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PCM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PCM1 using anti-PCM1 antibody. PCM1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PCM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
