Application
Flow cytometric analysis of K562 cells using PCM-1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PCM-1 antibody (C-eterm) in K562 cell line lysates (35ug/lane)| Product Name | PCM-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1 |
| Product description | PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.1) Balczon R., Bao L.J. Cell Biol. 124:783-793(1994) |
| Immunogen | This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1816-1845 amino acids from the C-terminal region of human PCM-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCM1 |
|---|---|
| Alternative Names | Pericentriolar material 1 protein PCM-1 hPCM-1 PCM1 |
| Molecular Weight(MW) | 229 kDa |
Application
Flow cytometric analysis of K562 cells using PCM-1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lymph reacted with PCM-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PCM-1 antibody (C-eterm) in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
