Application
Western blot Analysis: Whole cell lysates (lane 1: COS-7; lane 2: human M-cadherin overexpressed in COS-7 cells) were applied to SDS-PAGE and transferred to PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. Time: 30 sec).| Product Name | M-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [21G4] to M CadherinCadherins are a family of transmembrane glycoproteins that play a key role in Calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. M-Cadherin is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. |
| Immunogen | Recombinant full length protein - the extracellular domain of human M-Cadherin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 21G4 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: Use at a concentration of 0.05 μg/ml. IF: Use at a concentration of 5 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. (1ug/ml for AP/BCIP/NBT (MTT) detection 0.5ug/ml for HRP/ECL detection). Predicted molecular weight: 90 kDa. Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDH3 CDHM MCAD MRD3 CDH14 |
|---|---|
| Target | M Cadherin |
| Tissue Specificity | Extracellular domain of M Cadherin |
| Cellular Localization | Type 1 membrane protein |
Application
Western blot Analysis: Whole cell lysates (lane 1: COS-7; lane 2: human M-cadherin overexpressed in COS-7 cells) were applied to SDS-PAGE and transferred to PVDF membrane. The blot was probed with 0.5μg/ml for 1 hour at RT and developed by ECL (exp. Time: 30 sec).| Application Notes | ELISA: Use at a concentration of 0.05 μg/ml. IF: Use at a concentration of 5 μg/ml. WB: Use at a concentration of 0.5 - 1 μg/ml. (1ug/ml for AP/BCIP/NBT (MTT) detection 0.5ug/ml for HRP/ECL detection). Predicted molecular weight: 90 kDa. Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Preservative: 0.1% Sodium Azide, Constituents: 2X PBS, Polyethylene glycol, Sucrose |
