Application
Western blot analysis on THP-1 treated with LPS cell lysate using anti-MDA-5 antibody| Product Name | MDA5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody to MDA-5DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. MDA-5 is a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. |
| Immunogen | A synthetic peptide corresponding to residues in human MDA-5 was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR6743 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF: 1:250 - 500: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Hlcd IDDM19 MDA-5 MDA5 MGC133047 |
|---|---|
| Target | MDA-5 |
Application
Western blot analysis on THP-1 treated with LPS cell lysate using anti-MDA-5 antibody| Application Notes | ICC/IF: 1:250 - 500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 49% PBS, 50% Glycerol, 0.05% BSA, pH: 7.20 and 0.01% Sodium azide |
