Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MDC1 (Phospho-Ser513) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | MDC1 (phospho Ser513) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MDC1 (phospho Ser513)The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 513(E-R-S(p)-Q-A) derived from Human MDC1. |
| Modification | p-Ser513 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | mediator of DNA-damage checkpoint 1 NFBD1 MDC1 |
|---|---|
| Target | MDC1 (phospho Ser513) |
| Tissue Specificity | The antibody detects endogenous levels of MDC1 only when phosphorylated at serine 513. |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MDC1 (Phospho-Ser513) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
