Application
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells using at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.Application
Western Blot: MGMT Antibody (MT 23.2) - Analysis of MGMT (MT 23.2) antibody - Detection of MGMT in MCF7 lysate using at 1:1000 dilution.| Product Name | MGMT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [MT23.2] to MGMTMGMT is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. |
| Immunogen | Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli (ref 2) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | MT23.2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB: Use at a dilution of 1/1000 - 1/3000. Predicted molecular weight: 21.6 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Methylated-DNA--protein-cysteine methyltransferase METHYLGUANINE-DNA METHYLTRANSFERASE MGMT: O-6-methylguanine-DNA methyltransferase O-6-methylguanine-DNA-alkyltransferase |
|---|---|
| Target | MGMT |
| Tissue Specificity | This antibody is specific for MGMT. |
Application
Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) - Detection of MGMT (Green) in Hela cells using at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.
Application
Western Blot: MGMT Antibody (MT 23.2) - Analysis of MGMT (MT 23.2) antibody - Detection of MGMT in MCF7 lysate using at 1:1000 dilution.| Positive Control | CEM-CCRF cellsUse TK6 cells as a negative control |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000 - 1/3000. Predicted molecular weight: 21.6 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid with 0.1% sodium azide |
