| Product Name | MGMT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [MT 3.1] to MGMTCancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O6alkyl guanine position in DNA. O6-Methyl guanine-DNA Methyltransferase (MGMT) transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely be drug resistant. |
| Immunogen | Purified recombinant human MGMT protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MT3.1 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PWB |
| FACS: Use at an assay dependant dilution. IHC-P: Use at a concentration of 2 - 4 µg/ml. WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21.6 kDa). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Methylated-DNA--protein-cysteine methyltransferase METHYLGUANINE-DNA METHYLTRANSFERASE MGMT: O-6-methylguanine-DNA methyltransferase O-6-methylguanine-DNA-alkyltransferase |
|---|---|
| Target | MGMT |
| Application Notes | FACS: Use at an assay dependant dilution. IHC-P: Use at a concentration of 2 - 4 µg/ml. WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21.6 kDa). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.2% BSA and 0.09% sodium azide |
