Application
Confocal immunofluorescence analysis of Hela cells using anti-MLH1 mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using anti-MLH1 mAb.Application
Western blot analysis using anti-MLH1 mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.| Product Name | MLH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4C9C7] to MLH1This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009] |
| Immunogen | The immunogen corresponding to a region within amino acids 381 and 483 of MLH1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4C9C7 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Gene Synonyms | COCA2 HMLH1 HNPCC2 HNPCC FCC2 |
|---|---|
| Target | MLH1 |
| Molecular Weight(MW) | 85 kDa (note) |
Application
Confocal immunofluorescence analysis of Hela cells using anti-MLH1 mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using anti-MLH1 mAb.
Application
Western blot analysis using anti-MLH1 mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
