Application
Flow Cytometry: MLL-1 Antibody - Flow cytometric detection of MLL1. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 mcg/ml anti-MLL1 in a 150 mcl reaction. Isotype control (black), anti- MLL1 (red).Application
'Western Blot: MLL-1 Antibody - Detection of Human and Mouse MLL1 by Western Blot. Samples: Nuclear extract (50 mcg for WT and KO; 10 mcg for EF, EN and EC) from 293T cells that were transfected with MLL1 c-terminal 180 kDa fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kDa fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody: used at the indicated concentrations Detection: One second exposure with chemiluminescence'Application
'Western Blot: KMT2A/MLL Antibody - Nuclear extract (NE; 15 and 50 mcg) and whole cell lysate (WCL; 15 and 50 mcg) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody.'| Product Name | MLL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MLLA partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor. |
| Immunogen | Synthetic peptide: NRTSAGTSSSG VSNRKRKRKV FSPIRSEPRS PSHSMRTRSG RLSSSELSPL TPPSSVSSSL, corresponding to N terminal amino acids 720-780 of Human MLL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PIPWB |
| For IHC-P: Use at a dilution of 1:100 - 1:250. For IP: Use at a concentration of 1 - 4 μg/mg. For WB: Use at a dilution of 1:1,000 - 1:10,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TRX1 Q03164 HRX CXXC7 MLL ALL1 HTRX1 ALL-1 159555 MLL1A 4297 ALL 1 |
|---|---|
| Target | MLL |
| Tissue Specificity | human MLL1 protein |
| Cellular Localization | Nuclear |
Application
Flow Cytometry: MLL-1 Antibody - Flow cytometric detection of MLL1. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 mcg/ml anti-MLL1 in a 150 mcl reaction. Isotype control (black), anti- MLL1 (red).
Application
'Western Blot: MLL-1 Antibody - Detection of Human and Mouse MLL1 by Western Blot. Samples: Nuclear extract (50 mcg for WT and KO; 10 mcg for EF, EN and EC) from 293T cells that were transfected with MLL1 c-terminal 180 kDa fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kDa fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody: used at the indicated concentrations Detection: One second exposure with chemiluminescence'
Application
'Western Blot: KMT2A/MLL Antibody - Nuclear extract (NE; 15 and 50 mcg) and whole cell lysate (WCL; 15 and 50 mcg) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody.'| Positive Control | 293T cell lysate |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:100 - 1:250. For IP: Use at a concentration of 1 - 4 μg/mg. For WB: Use at a dilution of 1:1,000 - 1:10,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Tris citrate/phosphate, pH 7-8, containing 0.1% sodium azide |
