Application
Figure 1: Western blot analysis using anti-MLL monoclonal antiobdy against truncated MLL recombinant protein(1) and truncated GFP-MLL(aa3714-3969) transfected COS-7 cell lysate(2).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer(left) and esophagus cancer(right), showing nuclear weak staining with DAB staining using anti-MLL antibody.| Product Name | MLL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLL |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10F8D7 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALL1 CXXC7 HRX HTRX KMT2A MLL1 TRX |
|---|---|
| Alternative Names | MLL |
| Molecular Weight(MW) | 431764 Da |
| Function | Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis |
| Tissue Specificity | Heart, lung, brain and T- and B-lymphocytes. |
| Cellular Localization | Nucleus. MLL cleavage product C180: Nucleus. Note=Localizes to a diffuse nuclear pattern when not associated with MLL cleavage product N320 |
| Entrez Gene | 4297 |
|---|---|
| Nucleotide Accession | NP_005924.2 |
Application
Figure 1: Western blot analysis using anti-MLL monoclonal antiobdy against truncated MLL recombinant protein(1) and truncated GFP-MLL(aa3714-3969) transfected COS-7 cell lysate(2).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer(left) and esophagus cancer(right), showing nuclear weak staining with DAB staining using anti-MLL antibody.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of MLL(aa3751-3968) expressed in E. Coli. |
