Application
Immunohistochemical analysis of paraffin - embedded human lung cancer (top) and esophagus cancer (bottom) showing nuclear weak staining with DAB staining using MLL antibody.Application
Western blot analysis using anti MLL monoclonal antibody against truncated MLL - Trx recombinant protein (1) and truncated GFP - MLL (aa3714 - 3969) grasfected COS - 7 cell lsyate (2).| Product Name | MLL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1, ALL-1, Lysine N-methyltransferase 2A, HTRX1, MLL1A, WDSTS, MLL/GAS7, TET1-MLL |
| Product description | Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila).Eukaryotic RNA polymerase II mediates the synthesis of mature and functional messenger RNA. This is a multistep process, called the transcription cycle,that includes five stages: preinitiation, promoter, clearance, elongation and termination. Elongation is thought to be a critical stage for the regulation of gene expression. ELL (11-19 lysine-rich leukemia protein, also designated MEN) functions as an RNA polymerase II elongation factor that increases the rateof transcription by suppressing transient pausing by RNA polymerase II. Also, ELL is thought to regulate cellular proliferation. ELL is abundantly expressed in peripheral blood leukocytes, skeletal muscle, placenta and testis, and has lower expression in spleen, thymus, heart, brain, lung, kidney, liver and ovary.The gene encoding human ELL, which maps to chromosome 19p13.1, is one of several genes which undergo translocation with the MLL gene on chromo-some 11q23 in acute myeloid leukemia. MLL (myeloid/lymphoid leukemia,also designated ALL-1 and HRX) is a 430 kDa protein that regulates embryonal and hematopoietic development.1) Genet Couns. 2006;17(2):155-9. |
| Immunogen | Purified truncated recombinant MLL-Trx (aa3751-3968) is expressed in E.Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KMT2A |
|---|---|
| Alternative Names | ALL1 CXXC7 HRX HTRX MLL MLL1 TRX1 ALL-1 Lysine N-methyltransferase 2A HTRX1 MLL1A WDSTS MLL/GAS7 TET1-MLL |
Application
Immunohistochemical analysis of paraffin - embedded human lung cancer (top) and esophagus cancer (bottom) showing nuclear weak staining with DAB staining using MLL antibody.
Application
Western blot analysis using anti MLL monoclonal antibody against truncated MLL - Trx recombinant protein (1) and truncated GFP - MLL (aa3714 - 3969) grasfected COS - 7 cell lsyate (2).| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MLL monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
