Application
HRX Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HRX Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLL; ALL1; CXXC7; HRX; HTRX; KMT2A; MLL1; TRX1; Histone-lysine N-methyltransferase MLL; ALL-1; CXXC-type zinc finger protein 7; Lysine N-methyltransferase 2A; Trithorax-like protein; Zinc finger protein HRX; MLL cleavage product N320; N-terminal cleavage p |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FC |
| FC:1:10~50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALL1 CXXC7 HRX HTRX KMT2A MLL1 TRX |
|---|---|
| Alternative Names | MLL ALL1 CXXC7 HRX HTRX KMT2A MLL1 TRX1 Histone-lysine N-methyltransferase MLL ALL-1 CXXC-type zinc finger protein 7 Lysine N-methyltransferase 2A Trithorax-like protein Zinc finger protein HRX MLL cleavage product N320 N-terminal cleavage p |
| Molecular Weight(MW) | 431764 Da |
| Function | Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis |
| Tissue Specificity | Heart, lung, brain and T- and B-lymphocytes. |
| Cellular Localization | Nucleus. MLL cleavage product C180: Nucleus. Note=Localizes to a diffuse nuclear pattern when not associated with MLL cleavage product N320 |
Application
HRX Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | FC:1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
