| Product Name | MMP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP3Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. MMP3 is reported to be implicated in rheumatoid arthritis and some forms of cancer. |
| Immunogen | Synthetic peptide (Human) propeptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects bands of approximately 57 & 59 kDa. Is unsuitable for ELISA, IHC or IP. By immunoblotting against the reduced protein, the antibody reacts with bands at 59 kDa and 57 kDa (the proform), and not the active forms, thus anti-MMP3, propeptide region can be used to discriminate latent from activated MMP3. Anti-MMP3, propeptide region will also bind to the non-reduced protein, but less efficiently than to the reduced protein. MMP3 levels in quiescent cells and tissue are minimal, and stimulation of protein concentration is often needed to visualize the bands by immunoblotting. In addition, cell types differ greatly in the quantity of MMP3 produced. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | STR1 MGC126102 STMY SL1 STMY1 SL-1 MGC126103 185250 MGC126104 P08254 4314 MMP3 SL 1 |
|---|---|
| Target | MMP3 |
| Tissue Specificity | binds to stromelysin 1 and stromelysin 2, but does not crossreact with stromelysin 3 or other MMP family members (MMP1, MMP2, MMP3, MMP9, etc). |
| Cellular Localization | Secreted |
| Application Notes | WB: Use at a dilution of 1/1000. Detects bands of approximately 57 & 59 kDa. Is unsuitable for ELISA, IHC or IP. By immunoblotting against the reduced protein, the antibody reacts with bands at 59 kDa and 57 kDa (the proform), and not the active forms, thus anti-MMP3, propeptide region can be used to discriminate latent from activated MMP3. Anti-MMP3, propeptide region will also bind to the non-reduced protein, but less efficiently than to the reduced protein. MMP3 levels in quiescent cells and tissue are minimal, and stimulation of protein concentration is often needed to visualize the bands by immunoblotting. In addition, cell types differ greatly in the quantity of MMP3 produced. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
