Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MSH3 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MSH3 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A549 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MSH3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MSH3 (DNA mismatch repair protein Msh3)Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human MSH3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (83%), Rat (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DUP antibody MGC163306 antibody MGC163308 antibody MRP1 antibody MSH3 antibody mismatch repair protein 1 antibody divergent upstream protein antibody hMSH3 antibody DNA mismatch repair protein Msh3 antibody mutS homolog 3 (E. coli) antibody |
|---|---|
| Molecular Weight(MW) | 127 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MSH3 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MSH3 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A549 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
