Application
MSH3 antibody detects MSH3 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 5 % SDS-PAGE MSH3 antibody dilution: 1:1000| Product Name | MSH3 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MSH3 (mutS homolog 3 (E. coli))Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MSH3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DUP antibody MGC163306 antibody MGC163308 antibody MRP1 antibody MSH3 antibody mismatch repair protein 1 antibody divergent upstream protein antibody hMSH3 antibody DNA mismatch repair protein Msh3 antibody mutS homolog 3 (E. coli) antibody |
|---|---|
| Molecular Weight(MW) | 127 kDa (note) |
Application
MSH3 antibody detects MSH3 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 5 % SDS-PAGE MSH3 antibody dilution: 1:1000| Positive Control | A549 , H1299 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
