Application
WB analysis of HCT116 and 293 lysates using MSH6 antibody.Application
ICC/IF analysis of HeLa cells using MSH6 antibody at a dilution of 1:100.| Product Name | MSH6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3E1-B11-F12] to MSH6This gene encodes a protein similar to the MutS protein.In E. coli,the MutS protein helps in the recognition of mismatched nucleotides,prior to their repair.A highly conserved region of approximately 150 aa,called the Walker-A adenine nucleotide binding motif,exists in MutS homologs.The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated.Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer.[provided by RefSeq,Jul 2008] |
| Immunogen | Purified recombinant fragment of human MSH6 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3E1-B11-F12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | mutS homolog 6 (E. coli) HNPCC5 HSAP GTBP |
|---|---|
| Target | MSH6 |
| Molecular Weight(MW) | 160kDa (note) |
| Cellular Localization | Nucleus. |
Application
WB analysis of HCT116 and 293 lysates using MSH6 antibody.
Application
ICC/IF analysis of HeLa cells using MSH6 antibody at a dilution of 1:100.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
