Application
Immunohistochemical analysis of paraffin-embedded Hepatoma, using mtPAP antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using mtPAP antibody at 1:100 dilution.| Product Name | mtPAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1D3] to mtPAPThis antibody binds to mtPAP. The protein encoded by this gene (PAPD1) is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation. [from RefSeq] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ10486 mtPAP RP11305E6.3 PAPD1 RP11-305E6.3 |
|---|---|
| Target | mtPAP |
Application
Immunohistochemical analysis of paraffin-embedded Hepatoma, using mtPAP antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using mtPAP antibody at 1:100 dilution.| Positive Control | A549 , H1299 , HCT116 , MCF-7 |
|---|---|
| Application Notes | Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short term use, store at 4°C. For extended use, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS |
