Application
Sandwich ELISA analysis of purified breast cancer antigen protein using MUC1 antibody as a capture reagent and biotinylated Mouse anti Human MUC1, clone 6A4 as a detection reagent with purified breast cancer antigen. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.Application
Sandwich ELISA analysis of purified breast cancer antigen protein using as a capture reagent and biotinylated Mouse anti Human MUC1, clone VU-4H5 as a detection reagent with purified breast cancer antigen. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.| Product Name | MUC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [C595 (NCRC48)] to MUC1This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011] |
| Immunogen | Urinary MUC-1 mucin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | C595 (NCRC48) |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-FIHC-PWB |
| Pair for sandwich ELISA: GTX30742 MUC1 antibody [C595 (NCRC48)] for capture antibody, and GTX42237 MUC1 antibody [VU-4H5] for detection antibody.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MUC-1/X PEMT MUC-1/SEC mucin 1 cell surface associated PUM KL-6 CA 15-3 MUC1/ZD MUC-1 PEM EMA H23AG MAM6 CD227 |
|---|---|
| Target | MUC1 |
| Tissue Specificity | This antibody recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. |
Application
Sandwich ELISA analysis of purified breast cancer antigen protein using MUC1 antibody as a capture reagent and biotinylated Mouse anti Human MUC1, clone 6A4 as a detection reagent with purified breast cancer antigen. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.
Application
Sandwich ELISA analysis of purified breast cancer antigen protein using as a capture reagent and biotinylated Mouse anti Human MUC1, clone VU-4H5 as a detection reagent with purified breast cancer antigen. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.| Application Notes | Pair for sandwich ELISA: GTX30742 MUC1 antibody [C595 (NCRC48)] for capture antibody, and GTX42237 MUC1 antibody [VU-4H5] for detection antibody.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
