Application
IHC Image using - Detection of MUC1 by IHC in Human Breast Carcinoma| Product Name | MUC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [GP1.4] to MUC1This antibody recognizes the multiple protein epitope that is relatively insensitive to glycosylation. Therefore, it is very sensitive in the detection of episialin expression in different epithelial tissues. EMA = Epithelial Membrane antigen. |
| Immunogen | Human milk fat globule membranes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GP1.4 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method for 30 minutes at RT. Enzymatic predigestion of paraffin embedded tissues is not necessary but in some cases it may enhance the immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | mucin PEMT MUC1 PUM 4582 158340 P15941 EMA PEM H23AG MAM6 CD227 |
|---|---|
| Target | MUC1 |
| Tissue Specificity | Reacts with an antigen of 265-400 kD belonging to a heterogeneous group of heavily glycosylated proteins called human milk fat globule proteins. It stains both normal and neoplastic cells. Among normal epithelia, it reacts strongly with mammary epithelium and glandular epithelia but shows a patchy staining with squamous epithelium. |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Light Chain Type | kappa |
Application
IHC Image using - Detection of MUC1 by IHC in Human Breast Carcinoma| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method for 30 minutes at RT. Enzymatic predigestion of paraffin embedded tissues is not necessary but in some cases it may enhance the immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS pH 7.2, containing 0.05% sodium azide |
