| Product Name | MUC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [M10H6] to MUC1MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. |
| Immunogen | High molecular weight (more than 300 kDa) glycoprotein from human milk-fat globule molecule. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | M10H6 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC |
| ELISA: Use at an assay dependent dilution. Can be used in ELISA pairs with GTX28322, 10120, 10123 and 10115. IF: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. Immunohistochemical and immunofluorescent detection of neoplastic breast epithelium. Antibodies are working on frozen tissue sections. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | mucin PEMT MUC1 PUM 4582 158340 P15941 EMA PEM H23AG MAM6 CD227 |
|---|---|
| Target | MUC1 |
| Tissue Specificity | This products reacts with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosilated MUC1 prepared from tumor fluids or as a result of chemical treatment of †he human milk MUC1. This product does not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific Mabs and belongs to Mab cluster 1-4† according to ISOMB TD-4 classification.No cross-reactivity with egg white avidin. Specific to unglycosylated VNTR region |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Application Notes | ELISA: Use at an assay dependent dilution. Can be used in ELISA pairs with GTX28322, 10120, 10123 and 10115. IF: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. Immunohistochemical and immunofluorescent detection of neoplastic breast epithelium. Antibodies are working on frozen tissue sections. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
