| Product Name | MUC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [M2C5] to MUC1Detects underglycosylated and natural MUC1 protein . |
| Immunogen | High molecular weight (more than 300 kDa) glycoprotein from human milk-fat globule molecule |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | M2C5 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-P |
| Can be used for immunohistochemical staining of paraffin and frozen sections of breast,lung and ovarian tumor tissues. It may be used for staining of paraffin-embedded tissue sections fixed in neutral-buffered formalin and is compatible with commonly used histological fixatives. This antibody can be used in the form of an enzyme conjugate for development in ELISA sandwich assays of under-glycosylated tumor-associated MUC1 mucins in human serum. This is in conjunction with GTX28322 specific to the unglycosylated VNTR region of MUC1 as a capture antibody. This antibody has been tested by flowcytometry against primary mouse mammary epithelial cells, a mousemammary epithelial cell line and two human breast tumor cell lines (MCF-7 and SKBR3). No staining was detected in this flow cytometry experiment. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | mucin PEMT MUC1 PUM 4582 158340 P15941 EMA PEM H23AG MAM6 CD227 |
|---|---|
| Target | MUC1 |
| Tissue Specificity | Affinity = > 1.0 x10 8 Recognizes underglycosylated and natural MUC1 protein. Not cross reactive with normal serum proteins and human tissues of nonepithelial origin. |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Application Notes | Can be used for immunohistochemical staining of paraffin and frozen sections of breast,lung and ovarian tumor tissues. It may be used for staining of paraffin-embedded tissue sections fixed in neutral-buffered formalin and is compatible with commonly used histological fixatives. This antibody can be used in the form of an enzyme conjugate for development in ELISA sandwich assays of under-glycosylated tumor-associated MUC1 mucins in human serum. This is in conjunction with GTX28322 specific to the unglycosylated VNTR region of MUC1 as a capture antibody. This antibody has been tested by flowcytometry against primary mouse mammary epithelial cells, a mousemammary epithelial cell line and two human breast tumor cell lines (MCF-7 and SKBR3). No staining was detected in this flow cytometry experiment. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
