| Product Name | MUC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MUC2This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic MUC2 (23 aa) tandem repeat consensus sequence |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. For FFPE tissue sections high temperature antigen retriever (boiling tissue in 10 mM citrate buffer for 10 minutes) enhances the signal and may require further dilution of antibody.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | SMUC MLP MUC-2 mucin 2 oligomeric mucus/gel-forming |
|---|---|
| Target | MUC2 |
| Cellular Localization | Cytoplasmic, perinuclear staining of goblet cells. |
| Positive Control | Human colon carcinoma, LS 174 T colon cancer cell line |
|---|---|
| Application Notes | IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. For FFPE tissue sections high temperature antigen retriever (boiling tissue in 10 mM citrate buffer for 10 minutes) enhances the signal and may require further dilution of antibody.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 2-8°C. Do not freeze. |
| Storage Buffer | 10mM PO4, 200mM NaCl containing 1% BSA and 0.05% sodium azide |
