Application
NANOG antibody [GT3312] validation by siRNA knock-down. Upperpanel: NANOG antibody [GT3312] Lower panel: GAPDH antibody A. 30 µg Tera-2 whole cell lysate/extract B. 30 µg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells C. 30 µg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells D. 30 µg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells 10% SDS-PAGE NANOG antibody [GT3312] dilution: 1:1000 GAPDH antibody dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
NANOG antibody [GT3312] detects NANOG protein by western blot analysis. A. 30 µg human ESC whole cell lysate/extract 10% SDS-PAGE NANOG antibody [GT3312] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (20 µg of whole cell lysate) A: mouse ESC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | Nanog antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT3312] to Nanog (Nanog homeobox)Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT3312 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | ChimpanzeeChimpanzee (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NANOG antibody homeobox transcription factor Nanog-delta 48 antibody hNanog antibody homeobox protein NANOG antibody homeobox transcription factor Nanog antibody Nanog homeobox antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
NANOG antibody [GT3312] validation by siRNA knock-down. Upperpanel: NANOG antibody [GT3312] Lower panel: GAPDH antibody A. 30 µg Tera-2 whole cell lysate/extract B. 30 µg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells C. 30 µg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells D. 30 µg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells 10% SDS-PAGE NANOG antibody [GT3312] dilution: 1:1000 GAPDH antibody dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
NANOG antibody [GT3312] detects NANOG protein by western blot analysis. A. 30 µg human ESC whole cell lysate/extract 10% SDS-PAGE NANOG antibody [GT3312] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (20 µg of whole cell lysate) A: mouse ESC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
