Application
Western blot analysis of extracts of various cell lines, using NCK1 antibody.Application
Immunofluorescence analysis of A549 cell using NCK1 antibody. Blue: DAPI for nuclear staining.| Product Name | NCK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCK1The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010] |
| Immunogen | Recombinant protein of human NCK1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NCKalpha nck-1 NCK NCK adaptor protein 1 |
|---|---|
| Target | NCK1 |
| Molecular Weight(MW) | 43 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using NCK1 antibody.
Application
Immunofluorescence analysis of A549 cell using NCK1 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
