| Product Name | Nestin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2Q178] to Nestin - Neuronal MarkerNestin is a 200kD, class VI intermediate filament protein that is predominantly expressed in stem cells of the neural tube; its expression usually being absent from mature neuronal cells. Nestin is downregulated upon terminal neural differentiation and replaced by neurofilaments. Nestin is extensively used as a marker to identify CNS stem cells. NB: Nestin expression has been more recently discovered in non-neural stem cell populations, such as pancreatic islet progenitors as well as hematopoietic progenitors. |
| Immunogen | Full length native Nestin protein purified from embryonic rat spinal cord. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2Q178 |
| Host Species | Mouse |
| Tested Applications | EMICC/IFIHC-FIHC-PWB |
| For EM: Tissue fixed with 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4. For ICC: Use at a dilution of 1:10 - 1:100. Cells fixed with 4% paraformaldehyde buffered with 50mM sodium borate at pH 9.5. For IHC-P: Use at a dilution of 1:10 - 1:100. Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. For IHC-Fr: Use at a dilution of 1:10 - 1:100. Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. For WB: Use at an assay dependent dilution. Predicted molecular weight: 200 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nes Nestin 25491 |
|---|---|
| Target | Nestin |
| Cellular Localization | Cytoplasmic |
| Application Notes | For EM: Tissue fixed with 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4. For ICC: Use at a dilution of 1:10 - 1:100. Cells fixed with 4% paraformaldehyde buffered with 50mM sodium borate at pH 9.5. For IHC-P: Use at a dilution of 1:10 - 1:100. Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. For IHC-Fr: Use at a dilution of 1:10 - 1:100. Tissue fixed with 4% paraformaldehyde at pH 7.4 for light microscopy. For WB: Use at an assay dependent dilution. Predicted molecular weight: 200 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.09% sodium azide |
