WB
Nestin Western Blot. 20 ug of total protein from adult mouse brain in each lane. Lane A is Aves Labs' antibody (1:2000), showing a single, strong band at the correct molecular weight. Lane B uses a rabbit antibody from BD Transduction Labs showing a weak band at the same molecular weight, as well as another spurious band at about 145 kDa.IF-P
Photomicrograph of a tissue section through the periventricular zone of an e16 mouse brain (4.0% paraformaldehyde-fixed). Green is nestin immunoreactivity (1:1000 dilution). Blue is DAPI nuclear staining.IF-P
Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (1:500). Green is staining of the granule cells; blue is DAPI nuclear staining.ICC/IF
Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (red staining, 1:1000 dilution); green is β-Tubulin 3 staining using a rabbit antibody (1:500); blue is DAPI nuclear staining.| Product Name | Anti-Nestin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptides. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgY |
| Host Species | Chicken |
| Tested Applications | ICC/IFIF-PWB |
| WB:1:10000-1:20000 IF-P:1:1000-1:2000 ICC/IF:1000-1:2000 |
|
| Species Reactivity | MouseRat |
| Purification | Affinity purified |
| Gene Symbol | Nes |
|---|---|
| Gene Synonyms | RC2 Marc2 ESTM46 Ifaprc2 |
| Gene Full Name | nestin |
| Gene Summary | Predicted to enable CCR5 chemokine receptor binding activity and intermediate filament binding activity. Acts upstream of or within cell projection morphogenesis; negative regulation of neuron apoptotic process; and nervous system development. Located in cytoplasm and intermediate filament. Is expressed in several structures, including central nervous system; embryo mesenchyme; eye; foregut-midgut junction; and metanephros. Orthologous to human NES (nestin). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Nestin |
| Molecular Weight(MW) | 250kDa |
WB
Nestin Western Blot. 20 ug of total protein from adult mouse brain in each lane. Lane A is Aves Labs' antibody (1:2000), showing a single, strong band at the correct molecular weight. Lane B uses a rabbit antibody from BD Transduction Labs showing a weak band at the same molecular weight, as well as another spurious band at about 145 kDa.
IF-P
Photomicrograph of a tissue section through the periventricular zone of an e16 mouse brain (4.0% paraformaldehyde-fixed). Green is nestin immunoreactivity (1:1000 dilution). Blue is DAPI nuclear staining.
IF-P
Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (1:500). Green is staining of the granule cells; blue is DAPI nuclear staining.
ICC/IF
Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (red staining, 1:1000 dilution); green is β-Tubulin 3 staining using a rabbit antibody (1:500); blue is DAPI nuclear staining.| Application Notes | WB:1:10000-1:20000 IF-P:1:1000-1:2000 ICC/IF:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody. |
| Storage Buffer | Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative. |
