Application
Immunohistochemical analysis of Neuropilin 1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and (bottom left, right) at 5 µg/ml.Application
Neuropilin 1 antibody detects Neuropilin 1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Neuropilin 1 protein stained by Neuropilin 1 antibody diluted at 1:250. Green: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
IHC-Frozen: Day 14 embryonic mouse brain (hippocampus) fixed with 4% paraformaldehyde.Application
Western Blot analysis of Neuropilin 1 from mouse brain tissue.| Product Name | Neuropilin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Neuropilin 1Neuropilin 1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor and semaphorin family members. Neuropilin 1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. |
| Immunogen | Synthetic peptide: TDLDKKNTEIKIDET, corresponding to C terminal amino acids 813 - 827 of Rat Neuropilin 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| For IHC-Fr: Use at a dilution of 0.5 - 1.5 µg/ml. : |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | Neuropilin 1 Vascular endothelial cell growth factor165 receptor NRP Neuropilin1 Vascular endothelial cell growth factor 165 receptor VEGF165R neuropilin1 transmembrane receptor |
|---|---|
| Target | Neuropilin 1 |
| Molecular Weight(MW) | 130kD (note) |
| Tissue Specificity | recognizes a 130 kDa protein from mouse and rat brain tissue extract in Western blot, and is enriched in embryonic neural tissue. |
| Cellular Localization | Plasma membrane |
Application
Immunohistochemical analysis of Neuropilin 1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and (bottom left, right) at 5 µg/ml.
Application
Neuropilin 1 antibody detects Neuropilin 1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Neuropilin 1 protein stained by Neuropilin 1 antibody diluted at 1:250. Green: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
IHC-Frozen: Day 14 embryonic mouse brain (hippocampus) fixed with 4% paraformaldehyde.
Application
Western Blot analysis of Neuropilin 1 from mouse brain tissue.| Positive Control | Mouse or rat brain tissues , Human cervix tissue |
|---|---|
| Application Notes | For IHC-Fr: Use at a dilution of 0.5 - 1.5 µg/ml. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS containing 0.05% BSA and 0.05% sodium azide |
