Application
Human Tonsil stained with anti-NFkB p50 antibody| Product Name | NFkB p105/p50 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NFkB p50NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. NF-kB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino-terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy-terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL-1alpha and TNF-alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NF-kB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NF-kB has been associated with a number of inflammatory diseases while persistent inhibition of NF-kB leads to inappropriate immune cell development or delayed cell growth. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| For IHC-P: Neat. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686C01211 EBP-1 KBF1 MGC54151 NF-kappa-B NF-kappaB NFKB-p105 NFKB-p50 p105 p50 EBP 1 NFKB p50 NF kappaB NFKB p105 NF kappa B EBP1 NFKBp50 NFkappaB NFKBp105 |
|---|---|
| Target | NFkB p105/p50 |
| Cellular Localization | Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B). |
Application
Human Tonsil stained with anti-NFkB p50 antibody| Positive Control | Testis, tonsil and prostate |
|---|---|
| Application Notes | For IHC-P: Neat. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
