Application
NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 μg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 μg of NFYB antibody 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYB antibody at 1:500 dilution.Application
Sample (20 µg) A: HeLa nucleus 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NFYB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFYB (nuclear transcription factor Y, beta)The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Pig, Rhesus Monkey, BovineMouse (98%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CBF-A antibody CBF-B antibody HAP3 antibody NF-YB antibody NFYB antibody nuclear transcription factor Y subunit beta antibody Transcription factor NF-Y B subunit antibody CAAT box DNA-binding protein subunit B antibody CCAAT-binding transcription |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 μg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 μg of NFYB antibody 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYB antibody at 1:500 dilution.
Application
Sample (20 µg) A: HeLa nucleus 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa nucleus , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
