Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-Nog mouse monoclonal antibody.Application
Anti-Nog mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with NogApplication
Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody .Application
Noggin antibody (4C12) at 1:2000 + Recombinant human NogginApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.| Product Name | Noggin antibody [4C12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4C12] to NogginEssential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 28-232 of human noggin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4C12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SYNS1, NOG, SYM1 |
|---|---|
| Target | Noggin |
| Tissue Specificity | is specific for amino acids 28-232 of human noggin |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-Nog mouse monoclonal antibody.
Application
Anti-Nog mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with Nog
Application
Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody .
Application
Noggin antibody (4C12) at 1:2000 + Recombinant human Noggin
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
