Application
Anti-Notch1 mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1Application
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.| Product Name | NOTCH1 antibody [3E12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3E12] to Notch1Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3E12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NOTCH1, hN1, TAN1 |
|---|---|
| Target | Notch1 |
| Tissue Specificity | is specific for amino acids 2300-2556 of human NOTCH1 |
| Cellular Localization | Following proteolytical processing NICD is translocated to the nucleus. |
Application
Anti-Notch1 mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded endometrium using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded breast using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded pancreas using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded thyroid using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded kidney using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Notch1 mouse monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded colon using anti-Notch1 mouse monoclonal antibody.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
