Application
Immunohistochemistry-Paraffin: NrCAM Antibody [8E25] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NrCAM Antibody [8E25]. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.| Product Name | NrCAM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8E25] to NrCAMCell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | Human recombinant NrCAM |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 8E25 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| WB(1:500-1000): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NRCAM neuronal cell adhesion molecule |
|---|---|
| Target | NrCAM |
| Tissue Specificity | This antibody was selected for its ability to specifically detect human NrCAM. |
Application
Immunohistochemistry-Paraffin: NrCAM Antibody [8E25] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NrCAM Antibody [8E25]. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.| Application Notes | WB(1:500-1000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
