| Product Name | NSE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5A4] to NSE - Neuronal MarkerEnolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. |
| Immunogen | Recombinant full length protein (Human) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5A4 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/300. Antigen retrieval is not essential but may optimise staining. WB: Use at an assay dependent dilution. Detects a band of approximately 45 kDa. Can be used in an ELISA pair with GTX28328 (conjugate). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | NSE |
|---|---|
| Target | NSE |
| Cellular Localization | Cytoplasmic |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/300. Antigen retrieval is not essential but may optimise staining. WB: Use at an assay dependent dilution. Detects a band of approximately 45 kDa. Can be used in an ELISA pair with GTX28328 (conjugate). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
