| Product Name | NSE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal to Human Neuron - Specific EnolaseThis antibody reacts with the human 46 kD gamma enolase polypeptide. It also reacts with the gamma gamma enolase enzyme which is present in neurons, ganglion cells, and neuroendocrine (APUD) cells. |
| Immunogen | Purified NSE from Human Brain |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5E2 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-PWB |
| Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III (GTX28620). Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NSE |
|---|---|
| Target | Neuron-Specific Enolase |
| Cellular Localization | Cytoplasmic |
| Application Notes | Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III (GTX28620). Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.2% BSA and 0.09% sodium azide |
