Mouse monoclonal antibody [1D10H6] to Oct4This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010]
Immunogen
The immunogen corresponding to a region within amino acids 193 and 360 of Oct4 expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D10H6
Host Species
Mouse
Tested Applications
ELISAWB
ELISA: 1/10000.:
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
OTF3 OCT-3 OCT-4 OTF4 OCT4 POU5F1 OTF-3 OCT3
Target
Oct4
Molecular Weight(MW)
38.5 kDa (note)
Cellular Localization
Expressed in a diffuse and slightly punctuate pattern.
Application
Application
Western blot analysis using anti-Oct4 monoclonal antibody against recombinant Oct4 protein with Trx tag(1).
Application Notes
ELISA: 1/10000.:
Additional Information
Form
Liquid
Storage Instructions
Store at 4℃, for long term storage, store at -20℃.