Application
Flow cytometric analysis of Jurkat cells using Oct4 mouse mAb ( green) and negative control (purple).Application
Immunofluorescence analysis of NTERA-2 cells using Oct4 mouse mAb ( green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidinApplication
Western blot analysis using Oct4 mouse mAb against NTERA-2 (1) cell lysate.| Product Name | Oct4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7E7] to Oct4This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010] |
| Immunogen | The immunogen corresponding to human POU5F1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7E7 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OCT-3 OTF3 OCT3 OTF-3 OTF4 OCT-4 POU5F1 OCT4 |
|---|---|
| Target | OCT4 |
| Molecular Weight(MW) | 45 kDa (note) |
Application
Flow cytometric analysis of Jurkat cells using Oct4 mouse mAb ( green) and negative control (purple).
Application
Immunofluorescence analysis of NTERA-2 cells using Oct4 mouse mAb ( green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
Application
Western blot analysis using Oct4 mouse mAb against NTERA-2 (1) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
