| Product Name | P Glycoprotein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [C494] to P GlycoproteinP Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas. |
| Immunogen | Recombinant full length protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | C494 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC/IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:20 or greater, for Biotin-based detection systems. For optimal stainning, the primary antibody should be incubated 20-60 minutes at room temperature. No pre-treatment required. Note: Unequivocal plasma membrane patterns of immunostaining represent true P Glycoprotein expression. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the Pyruvate Carboxylase cross-reactive epitope rather than positive staining for P Glycoprotein. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 141 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABC20 CD243 GP170 MDR1 |
|---|---|
| Target | P Glycoprotein |
| Tissue Specificity | This antibody is specific for the MDR1 isoform of P Glycoprotein. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. |
| Cellular Localization | Cell Membrane |
| Positive Control | Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretory epithelium). |
|---|---|
| Application Notes | ICC/IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:20 or greater, for Biotin-based detection systems. For optimal stainning, the primary antibody should be incubated 20-60 minutes at room temperature. No pre-treatment required. Note: Unequivocal plasma membrane patterns of immunostaining represent true P Glycoprotein expression. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the Pyruvate Carboxylase cross-reactive epitope rather than positive staining for P Glycoprotein. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 141 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS containing 1% BSA and 0.1% sodium azide |
