| Product Name | P Glycoprotein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [JSB - 1] to P GlycoproteinP Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas. |
| Immunogen | Multidrug-resistant Chinese hamster ovary cell line (CHrC5). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | JSB-1 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PIPWB |
| ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/40 or greater, for Biotin-based detection systems. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 141 kDa. For optimal stainning, the primary antibody should be incubated 20-60 minutes at room temperature. Sections should be pretreated with an antigen unmasking solution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMammalian species |
| Concentration | 1mg/ml |
| Gene Synonyms | ABC20 CD243 GP170 MDR1 |
|---|---|
| Target | P Glycoprotein |
| Tissue Specificity | This antibody is specific for the MDR1 isoform of P Glycoprotein. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P glycoprotein. |
| Cellular Localization | Cell Membrane |
| Positive Control | Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Human liver (positive staining detected along luminal surfaces of bilecanaliculi) or human colon (positive staining localized to luminal surface of secretaoryepithelium). |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/40 or greater, for Biotin-based detection systems. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 141 kDa. For optimal stainning, the primary antibody should be incubated 20-60 minutes at room temperature. Sections should be pretreated with an antigen unmasking solution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 1% BSA, 0.01M PBS |
