Application
ICC/IF analysis of methanol-fixed HeLa cells using PAK1/2/3 (phospho Thr423/Thr402/Thr421) antibody (red) and DAPI (blue).Application
IHC-P analysis of human breast carcinoma tissue using PAK1/2/3 (phospho Thr423/Thr402/Thr421) antibody.Application
IHC-P analysis of human breast carcinoma tissue using PAK1/2/3 (phospho Thr423/Thr402/Thr421) antibody pre-incubated with blocking peptide.| Product Name | PAK1/2/3 (phospho Thr423/Thr402/Thr421) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PAK1/2/3 (phospho Thr423/Thr402/Thr421)This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 423. |
| Modification | p-Thr423/Thr402/Thr421 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| IHC: 1:50-1:100 IF:1:100-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PAKalpha PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 |
|---|---|
| Target | PAK1 (phospho Thr423/402/421) |
| Tissue Specificity | This antibody detects endogenous levels of PAK1/PAK2/PAK3 only when phosphorylated at threonine 423/402/421. |
Application
ICC/IF analysis of methanol-fixed HeLa cells using PAK1/2/3 (phospho Thr423/Thr402/Thr421) antibody (red) and DAPI (blue).
Application
IHC-P analysis of human breast carcinoma tissue using PAK1/2/3 (phospho Thr423/Thr402/Thr421) antibody.
Application
IHC-P analysis of human breast carcinoma tissue using PAK1/2/3 (phospho Thr423/Thr402/Thr421) antibody pre-incubated with blocking peptide.| Application Notes | IHC: 1:50-1:100 IF:1:100-1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
