Application
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] ; Lane 2: PAPP A antibody [3C8] ; Lane 3: PAPP A antibody [5H9] ; Lane 4: PAPP A antibody [11E4] ; Lane 5: PAPP A antibody [10A5] ; Lane 6: PAPP A antibody [3C8] ; Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] and PAPP A antibody [3C8]| Product Name | PAPP A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [10A5] to PAPP APregnancy-Assosiated Plasma Protein A (PAPPA) is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. |
| Immunogen | Purified human PAPPA antigen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 10A5 |
| Host Species | Mouse |
| Tested Applications | ELISAIAWB |
| ELISA: Use at an assay dependent dilution. Can be used in immunoassay for the detection of human PAPPA antigen as an antibody pair with GTX10194 Mouse monoclonal [3C8] to PAPPA for coating. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. Recognizes heterotetrameric complex in Western Blotting after SDS-electrophoresis in non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | ASBABP2 DIPLA1 IGFBP-4ase PAPA PAPP-A PAPPA1 PAPP A IGFBP 4ase PAPPA IGFBP4ase |
|---|---|
| Target | PAPP A |
Application
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] ; Lane 2: PAPP A antibody [3C8] ; Lane 3: PAPP A antibody [5H9] ; Lane 4: PAPP A antibody [11E4] ; Lane 5: PAPP A antibody [10A5] ; Lane 6: PAPP A antibody [3C8] ; Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] and PAPP A antibody [3C8]| Application Notes | ELISA: Use at an assay dependent dilution. Can be used in immunoassay for the detection of human PAPPA antigen as an antibody pair with GTX10194 Mouse monoclonal [3C8] to PAPPA for coating. IA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. Recognizes heterotetrameric complex in Western Blotting after SDS-electrophoresis in non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
