Application
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] ; Lane 2: PAPP A antibody [3C8] ; Lane 3: PAPP A antibody [5H9] ; Lane 4: PAPP A antibody [11E4] ; Lane 5: PAPP A antibody [10A5] ; Lane 6: PAPP A antibody [3C8] ; Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] and PAPP A antibody [3C8]| Product Name | PAPP A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [11E4] to PAPP APAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al). |
| Immunogen | Full length native protein (purified) (Human) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 11E4 |
| Host Species | Mouse |
| Tested Applications | EIAELISAWB |
| ELISA: Use at an assay dependent dilution. EIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 129 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | PAPPA DIPLA1 5069 IGFBP-4ase ASBABP2 PAPPA1 PAPP A PAPA PAPP-A IGFBP4ase Q13219 176385 IGFBP 4ase |
|---|---|
| Target | PAPP A |
| Tissue Specificity | Specific to the proMBP component of the heterotetramer |
Application
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] ; Lane 2: PAPP A antibody [3C8] ; Lane 3: PAPP A antibody [5H9] ; Lane 4: PAPP A antibody [11E4] ; Lane 5: PAPP A antibody [10A5] ; Lane 6: PAPP A antibody [3C8] ; Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] and PAPP A antibody [3C8]| Application Notes | ELISA: Use at an assay dependent dilution. EIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 129 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
