Application
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] biotinylated; Detection: PAPP A antibody [10H9] labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.Application
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] biotinylated; Detection: PAPP A antibody [3C8] labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.| Product Name | PAPP A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4G11] to PAPP APregnancy-Assosiated Plasma Protein A (PAPPA) is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. |
| Immunogen | Purified human PAPPA antigen |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 4G11 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. Can be paired for ELISA with GTX10194 Mouse monoclonal [3C8] to PAPPA and GTX10195 Mouse monoclonal [10H9] to PAPPA . WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. GTX10192 recognizes heterotetrameric complex in Western Blotting after SDS-electrophoresis in non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | ASBABP2 DIPLA1 IGFBP-4ase PAPA PAPP-A PAPPA1 PAPP A IGFBP 4ase PAPPA IGFBP4ase |
|---|---|
| Target | PAPP A |
Application
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] biotinylated; Detection: PAPP A antibody [10H9] labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.
Application
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] biotinylated; Detection: PAPP A antibody [3C8] labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.| Application Notes | ELISA: Use at an assay dependent dilution. Can be paired for ELISA with GTX10194 Mouse monoclonal [3C8] to PAPPA and GTX10195 Mouse monoclonal [10H9] to PAPPA . WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. GTX10192 recognizes heterotetrameric complex in Western Blotting after SDS-electrophoresis in non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
