Application
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] biotinylated; Detection: PAPP A antibody [3C8] labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.Application
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] ; Lane 2: PAPP A antibody [3C8] ; Lane 3: PAPP A antibody [5H9] ; Lane 4: PAPP A antibody [11E4] ; Lane 5: PAPP A antibody [10A5] ; Lane 6: PAPP A antibody [3C8] ; Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] and PAPP A antibody [3C8]| Product Name | PAPP A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3C8] to PAPP APregnancy-associated plasma protein A (PAPPA) is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. |
| Immunogen | Purified human PAPPA antigen |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3C8 |
| Host Species | Mouse |
| Tested Applications | ELISAIAWB |
| ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. Can be paired for ELISA with GTX10192 Mouse monoclonal [4G11] to PAPPA and GTX10193 Mouse monoclonal [10A5] to PAPPA . Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | ASBABP2 DIPLA1 IGFBP-4ase PAPA PAPP-A PAPPA1 PAPP A IGFBP 4ase PAPPA IGFBP4ase |
|---|---|
| Target | PAPP A |
Application
Calibration curve for PAPP-A sandwich immunoassay. Capture: PAPP A antibody [4G11] biotinylated; Detection: PAPP A antibody [3C8] labeled with stable Eu3+ -chelate; Antigen: htPAPP-A; Mixture of antibodies and antigen was incubated for 30 minutes at room temperature in streptavidin-coated plates.
Application
Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blot. Lane 1: PAPP A antibody [18A10] ; Lane 2: PAPP A antibody [3C8] ; Lane 3: PAPP A antibody [5H9] ; Lane 4: PAPP A antibody [11E4] ; Lane 5: PAPP A antibody [10A5] ; Lane 6: PAPP A antibody [3C8] ; Lanes 1-4: reducing conditions; Lanes 5-6: non-reducing conditions, heterotetrameric complex was detected by PAPP A antibody [10A5] and PAPP A antibody [3C8]| Application Notes | ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent dilution. Can be paired for ELISA with GTX10192 Mouse monoclonal [4G11] to PAPPA and GTX10193 Mouse monoclonal [10A5] to PAPPA . Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
