Application
Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 ug/ml.Application
Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 ug/ml.| Product Name | PARL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PARLPARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL’s association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer’s disease through its associations with presenilin-1 and -2. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Multiple isoforms of PARL are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Presenilinassociated rhomboidlike protein PSNENIP2 PARL PSARL Presenilin-associated rhomboid-like protein PSARL1 |
|---|---|
| Target | PARL |
| Tissue Specificity | This antibody is specific for PARL |
Application
Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 ug/ml.
Application
Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85382-PEP - PARL Peptide Lysates: Cat. No. GTX27901 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
