Application
Cell lysates of 293T and U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | PD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4F12] to PD1PDCD1 is an immunoreceptor that belongs to the immunoglobulin (Ig) superfamily, also known as Programmed Cell Death 1. It is expressed on activated T cell, B cells, myeloid cells and a subset of thymocytes. PDCD1 interacts with expressed PDL1 and PDL2 to inhibit the proliferation and activation of T cells and B cells. Both PDL1 and PDL2 are expressed in a wide variety of normal tissues including heart, lung, placenta, spleen and kidney. PDCD1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases |
| Immunogen | Recombinant human PDCD1 (21-167aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4F12 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:500 ~ 1,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD279 hPD1 PD1 hPD-1 PDCD1 Programmed cell death1 hPDl Programmed cell death 1 hPD-l hPD 1 hPD l |
|---|---|
| Target | PD1 |
| Light Chain Type | kappa |
Application
Cell lysates of 293T and U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:500 ~ 1,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
