Application
Western blot of PDE4A using| Product Name | PDE4A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE4AEnzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling. |
| Immunogen | Synthetic peptide corresponding to the C-terminal region of PDE4A (common to all PDE4A proteins). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at a dilution of 1/250. IF: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant). Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 5141 PDE4 DPDE2 P27815 600126 PDE4A PDE46 |
|---|---|
| Target | PDE4A |
| Tissue Specificity | This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), A5 (109 kDa), A8 (106 kDa), Ax (102 kDa) and a 76 kDa testis specific A variant. |
| Cellular Localization | Cell Membrane |
Application
Western blot of PDE4A using| Application Notes | ICC: Use at a dilution of 1/250. IF: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
