Application
Confocal micrograph of rat testis. A: Section labeled with antibody B: Section labeled with antibodyApplication
Western blot of PDE4A8 using| Product Name | PDE4A8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE4A8The PDE4A8 is a testis germ cell-specific PDE4A variant. There are two PDE4A variants identified in rat testis (PDE4A8 and an un-characterized 76 kDa PDE4A protein). Spermatogenesis is accompanied by expression of a varied repertoire of PDE4 enzymes at different stages of cell differentiation that presumably perform specialized functions. Confocal microscopic examination revealed the presence of PDE4A8 in primary and secondary spermatids but not in mature spermatozoa. PDE4A8 inhibition results in increased apoptosis of the germ cells accompanied with reduction in the number of mature spermatozoa. |
| Immunogen | N-terminus PDE4A8 synthetic cyclic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Predicted molecular weight: 106 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 5141 PDE4 PDE4A8 DPDE2 P27815 600126 PDE4A PDE46 |
|---|---|
| Target | PDE4A8 |
| Tissue Specificity | Does not cross react with other PDE4A variants including the novel 76 kDa testis-specific PDE4A protein. Does not cross react with PDE4B, C or D proteins. |
Application
Confocal micrograph of rat testis. A: Section labeled with antibody B: Section labeled with antibody
Application
Western blot of PDE4A8 using| Application Notes | ICC: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Predicted molecular weight: 106 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Thimerosal / Merthiolate |
