Application
Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/ml PDGFRalpha [pY742] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and bands were detected. The data show that only the peptide corresponding to PDGFRalpha [pY742] blocks the antibody signal, thereby demonstrating the specificity of the antibody.| Product Name | PDGF Receptor alpha (phospho Tyr742) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDGF Receptor alpha ( phospho Y742 )Platelet derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo- or heterodimerization upon ligand binding. The phosphorylation of tyrosine residues in the ligand-activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also leads to activation of the Ras, Raf and ERK 1+2 pathway. Tyrosine 742 in PDGFR alpha is a binding site for PI3 kinase. |
| Immunogen | Synthetic phospho peptide that contains Tyrosine 742 (Human). |
| Modification | p-Tyr742 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.35 - 1 μg/ml. Detects a band of approximately 170-185 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD140A MGC74795 PDGFR2 RHEPDGFRA PDGFR alpha (phospho Y742) PDGFR alpha (phospho Tyr742) PDGFR alpha (pY742) PDGFR alpha (pTyr742) PDGFR alpha phospho Y742 PDGFR alpha phospho Tyr742 Phospho PDGFR alpha (Y742) Phospho PDGFR alpha (Tyr742) Phos |
|---|---|
| Target | PDGFR alpha |
| Tissue Specificity | PDGFR beta (50% homologous) has not been tested, but is not expected to react. |
| Cellular Localization | Cell Membrane and Cytoplasmic. |
Application
Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/ml PDGFRalpha [pY742] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and bands were detected. The data show that only the peptide corresponding to PDGFRalpha [pY742] blocks the antibody signal, thereby demonstrating the specificity of the antibody.| Positive Control | NIH3T3 cells +/- PDGF |
|---|---|
| Application Notes | WB: Use at a concentration of 0.35 - 1 μg/ml. Detects a band of approximately 170-185 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
